Dynamic monitoring of morphological and physiological changes in single cells following photodynamic therapy (PDT)
Bibliographic record
Abstract
The standard method of performing investigations in cell biology consists of studying the behavior of average populations to extract information of the events occurring at a cellular and molecular level. Despite the unquestionable amount of knowledge accumulated with this approach, a closer look at the behavior in a single-cell and even subcellular scale is becoming more desirable as a way to study rare event populations. This becomes possible with the advent of an increasing number of fluorescent probes, which are compatible with cell viability and permit the monitoring of the morphological and physiological changes of a single cell following a particular challenge. The number of observed events can be kept large to ensure adequate population statistics. Changes associated with morphological and physiological responses are encoded with fluorescence parameters such as intensity, wavelength and spatial localization variations and fluorescence lifetime. In addition to this, following a particular challenge, confocal microscopy is a powerful tool to track these changes over time. It also provides a method of following the behavior of a large number of cells even at high magnifications, due to its versatility and automatization capabilities.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".