Microwave Irradiation of PC3 Cells at Constant Culture Temperature Alters the Incorporation of BODIPY into Cells and Reduction of MTT
Bibliographic record
Abstract
Abstract The present study investigated the impact of 10 W microwave irradiation at 2.45 GHz on the cell viability and membrane properties of human prostate cancer PC3 cells, while the temperature of the cell cultures was maintained at 37±2 °C through simultaneous cooling. Microwave irradiation under these conditions did not induce apoptosis, nor otherwise lead to loss of cell viability, as indicated by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), Annexin–V, and propidium iodide assays. Interestingly however, incorporation of BODIPY (4,4‐difluoro‐5‐methyl‐4‐bora‐3a,4a‐diaza‐ s ‐indacene) 3‐dodecanoic acid into the cell membrane was facilitated by microwave treatment at constant temperature. Also, constant temperature microwave treatment significantly increased the rate of reduction of MTT. Taken together, these results suggest that constant temperature microwave, while non‐lethal to PC3 cells, may alter membrane properties.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".