Environmental <scp>DNA</scp> metabarcoding: Transforming how we survey animal and plant communities
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Machine scores (provisional)
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
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- Teacher spread
- 0.192 · how far apart the two teachers sit on this one work
- Validation status
score_only:v0-immature-baseline· verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it
Abstract
The genomic revolution has fundamentally changed how we survey biodiversity on earth. High-throughput sequencing ("HTS") platforms now enable the rapid sequencing of DNA from diverse kinds of environmental samples (termed "environmental DNA" or "eDNA"). Coupling HTS with our ability to associate sequences from eDNA with a taxonomic name is called "eDNA metabarcoding" and offers a powerful molecular tool capable of noninvasively surveying species richness from many ecosystems. Here, we review the use of eDNA metabarcoding for surveying animal and plant richness, and the challenges in using eDNA approaches to estimate relative abundance. We highlight eDNA applications in freshwater, marine and terrestrial environments, and in this broad context, we distill what is known about the ability of different eDNA sample types to approximate richness in space and across time. We provide guiding questions for study design and discuss the eDNA metabarcoding workflow with a focus on primers and library preparation methods. We additionally discuss important criteria for consideration of bioinformatic filtering of data sets, with recommendations for increasing transparency. Finally, looking to the future, we discuss emerging applications of eDNA metabarcoding in ecology, conservation, invasion biology, biomonitoring, and how eDNA metabarcoding can empower citizen science and biodiversity education.
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The record
- Venue
- Molecular Ecology
- Topic
- Environmental DNA in Biodiversity Studies
- Field
- Environmental Science
- Canadian institutions
- Université Laval
- Funders
- European Social FundCenter for Sponsored Coastal Ocean ResearchNatural Environment Research CouncilNational Science FoundationStrategic Environmental Research and Development ProgramNERC Biomolecular Analysis FacilitySchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen ForschungBangor UniversitySight Research UKNational Oceanic and Atmospheric AdministrationEuropean Cooperation in Science and TechnologyLlywodraeth CymruU.S. Department of DefenseEcological Society of America
- Keywords
- BiologyEnvironmental DNAMolecular ecologyDNAEvolutionary biologyEcologyComputational biologyGeneticsBiodiversityPopulation
- Has abstract in OpenAlex
- yes