Stable isotopes as tracers in aquatic ecosystems
Bibliographic record
Abstract
The addition of stable isotopes (SI) of 13 C and 15 N has been used to study several aquatic processes, thus avoiding environmental disturbance by the observer. This approach, employed for the last three decades, has contributed to expanding our knowledge of food-web ecology and nutrient dynamics in aquatic systems. Currently, SI addition is considered a powerful complementary tool for studying several ecological and biogeochemical processes at the whole-aquatic-ecosystem scale, which could not be addressed otherwise. However, their contributions have not been considered jointly nor have they been evaluated with a view to assessing the reliability and scope of their results from an ecosystem perspective. We intend to bridge this gap by providing a comprehensive review (78 scientific publications reporting in situ 13 C/ 15 N additions at the whole-aquatic-ecosystem scale) addressing the main results arising from their use as tracers. Specifically, we focus on: (i) reasons for SI additions at the whole-ecosystem scale to study ecological processes, (ii) the paradigms resulting from its use and the insights achieved, (iii) uncertainties and drawbacks arising from these SI addition experiments, and (iv) the potential of this approach for tackling new paradigms. SI tracer addition at the ecosystem scale has provided new functional insights into numerous ecological processes in aquatic sciences (importance of subsidies in lakes; heterotrophy dominance in benthic food webs in lakes, wetlands and estuaries; the decrease in N removal efficiency in most aquatic ecosystems due to anthropogenic alteration; the recognition of hyporheic zones and floodplains as hot spots for stream denitrification; and high rates of internal N recycling in tidal freshwater marshes). However, certain constraints such as the high cost of isotopes, the maintenance of the new isotopic steady state, and avoidance of biomass changes in any compartment or pool during tracer addition bear witness to the difficulties of applying this approach to all fields of aquatic ecology and ecosystems. The future development of this approach, rather than expanding to larger and complex aquatic ecosystems, should include other stable isotopes such as phosphorus (P 18 O 4 ).
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.011 | 0.032 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; both teacher heads agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".