Abiotic stress tolerance and ABA responses of transgenic <i>Glycine max</i> plants with modulated RACK1 expression
Bibliographic record
Abstract
The receptor for activated C kinase 1 (RACK1) belongs to a protein subfamily that contains a tryptophan-aspartic acid-domain (WD) repeat structure. In this study, a soybean RACK1 gene (GmRACK1) was cloned. The amino acid sequence of GmRACK1 had seven WD repeats, which contained typical glycine-histidine (GH) and WD dipeptides. Tissue-specific expression showed that GmRACK1 is expressed at differential levels in all examined tissues and strongly down-regulatd by abscisic acid (ABA) and abiotic stress. Subcellular localization suggested that GmRACK1 is located in the plasma membrane, cytoplasm, and nucleus. In response to drought and salt stress, the GmRACK1-RNAi lines showed significantly higher dry weight of whole plants, chlorophyll content, and survival rate of soybean seedlings than wild-type and homozygous (OE) lines. GmRACK1-RNAi plants were observed to be more sensitive to ABA-mediated seed germination and root growth. Furthermore, we found that the level of ABA and transcript levels of stress-responsive genes were clearly up-regulated under drought and salt stress conditions in GmRACK1-RNAi plants. Consistent with the accumulation of reactive oxygen species (ROS), elevated electrolyte leakage and malondialdehyde, and lower expression of ROS-scavenging genes were found in the GmRACK1-OE lines. The GmRACK RNAi lines exhibited increased tolerance to drought and salt stress compared with the wild-type and OE lines. In addition, yeast two-hybrid assays showed that GmRACK1 could interact with WNK8, eIF6, and RbohC-N. These results therefore indicate that GmRACK1 responds to drought and salt stress through ABA signaling and the regulation of cellular ROS production in plants.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".