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Record W2768047557 · doi:10.1096/fj.201700552r

The cooling compound icilin attenuates autoimmune neuroinflammation through modulation of the T‐cell response

2017· article· en· W2768047557 on OpenAlexafffund
Benjamin W. Ewanchuk, Euan R.O. Allan, Amy L. Warren, Rithwik Ramachandran, Robin M. Yates

Bibliographic record

VenueThe FASEB Journal · 2017
Typearticle
Languageen
FieldNeuroscience
TopicIon Channels and Receptors
Canadian institutionsWestern UniversityUniversity of Calgary
FundersCanadian Institutes of Health ResearchMultiple Sclerosis Society of CanadaHealth Research Board
KeywordsNeuroinflammationTRPM8Transient receptor potential channelImmunologyExperimental autoimmune encephalomyelitisT cellInflammationChemistryPharmacologyReceptorBiologyImmune systemTRPV1Biochemistry

Abstract

fetched live from OpenAlex

The synthetic supercooling drug, icilin, and its primary receptor target, the cation channel transient receptor potential (TRP) melastatin‐8 (TRPM8), have been described as potent negative regulators of inflammation in the colon. The aim of this study was to determine whether the anti‐inflammatory action of icilin could potentially be used to treat autoimmune neuroinflammatory disorders, such as multiple sclerosis (MS). During experimental autoimmune encephalomyelitis (EAE)—a CD4 + T cell‐driven murine model of MS—we found that both wild‐type (WT) and TRPM8‐deficient EAE mice were protected from disease progression during icilin treatment, as evidenced by delays in clinical onset and reductions in neuroinflammation. In vitro , icilin potently inhibited the proliferation of murine and human CD4 + T cells, with the peripheral expansion of autoantigen‐restricted T cells similarly diminished by the administration of icilin in mice. Attenuation of both TRPM8 –/– and TRP ankyrin‐1 –/– T‐cell proliferation by icilin was consistent with the WT phenotype, which suggests a mechanism that is independent of these channels. In addition, icilin treatment altered the expressional profile of activated CD4 + T cells to one that was indicative of restricted effector function and limited neuroinflammatory potential. These findings identify a potent antiinflammatory role for icilin in lymphocyte‐mediated neuroinflammation and highlight clear pleiotropic effects of the compound beyond classic TRP channel activation.—Ewanchuk, B. W., Allan, E. R. O., Warren, A. L., Ramachandran, R., Yates, R. M. The cooling compound icilin attenuates autoimmune neuroinflammation through modulation of the T‐cell response. FASEB J. 32,1236‐1249 (2018). www.fasebj.org

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesScience and technology studies
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.032
Threshold uncertainty score0.997

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0040.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.045
GPT teacher head0.281
Teacher spread0.236 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations15
Published2017
Admission routes2
Has abstractyes

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