Assessment of antimicrobial and wound healing effects of Brevinin-2Ta against the bacterium<i>Klebsiella pneumoniae</i>in dermally-wounded rats
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Bibliographic record
Abstract
// Siqin Liu 1, * , Qilin Long 1, * , Yang Xu 2 , Jun Wang 2 , Zhongwei Xu 3 , Lei Wang 1 , Mei Zhou 1 , Yuxin Wu 1 , Tianbao Chen 1 and Chris Shaw 1 1 Natural Drug Discovery Group, School of Pharmacy, Queen’s University Belfast, United Kingdom 2 Department of General Surgery, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China 3 Center Laboratory of Logistics University of Chinese People’s Armed Police Forces, Tianjin, China * These authors contributed equally to this work and should be considered as co-first authors Correspondence to: Yuxin Wu, email: yuxin.wu@qub.ac.uk Keywords: AMP; brevinine; inflammation; angiogenesis; Klebsiella pneumoniae Received: August 13, 2017 Accepted: November 16, 2017 Published: November 30, 2017 ABSTRACT Antimicrobial peptides (AMPs) are regarded as promising alternatives for antibiotics due to their inherent capacity to prevent microbial drug resistance. Amphibians are rich source of bioactive molecules, which provide numerous AMPs with various structures as drug candidates. Here, we isolated and identified a novel AMP Brevinin-2Ta (B-2Ta) from the skin secretion of the European frog, Pelophylax kl. esculentus . In vitro studies revealed that it showed broad antimicrobial activities against S. aureus , E. coli and C. albicans with low cytotoxicity to erythrocytes. Furthermore, we examined the anti-inflammation effect in vivo by using Klebsiella pneumoniae -infected Sprague-Dawley (SD) rats. The wound closure outcomes revealed that B-2Ta effectively restrained the bacterial infection at a dose of 10 times minimal inhibitory concentration (MIC) during the 14 days of the wound healing process. Ultra-structure analyses showed that B-2Ta caused structural damage to the microorganism, and bacterial culture found that the number of microbes was significantly reduced by the end of treatment. Immunohistochemistry for the inflammatory marker IL-10 and the endothelial cell marker CD31 suggested positive effects on inflammatory status and epithelial migration and angiogenesis following treatment of the infected granulation tissues with B-2Ta. These results exhibited the continuous phase of inflammation reduction and wound healing acceleration in the B-2Ta-modulated re-epithelialisation of K. pneumoniae infected rats. Taken together, these data demonstrated that B-2Ta has great potential to be developed as antibacterial agents in clinic.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it