Identification of Mycoplasma Muris Isolated from Vaginal Samples of NIH Mice
Bibliographic record
Abstract
Mycoplasma muris PCR New strain 16S rRNA sequences NIH MiceMycoplasma muris (M.M) is a small pathogenic bacterium that lives in the female mouse genital tract.Mycoplasma muris may have harmful effects on the reproductive health of female.This research was performed to optimize the detection of M. muris in NIH mice in the Department of Animal Breeding, Razi Vaccine and Research Institute, Iran.In this cross-sectional study, 29 vaginal samples of NIH mice were selected through simple random sampling.For detection of the mycoplasma, the vaginal tissue removal of samples was done.First, samples were crushed using mortar and pestle with PBS 1ml, then were cultured in the PPLO broth and incubated at 37°C for 24h, they were passed through 0.45 μm pore-size filters and inoculated into specific PPLO broth and agar media for 3-4 weeks.In the next section, the PCR test was used with primers of 16S rRNA gene of M. muris.From 29 tested samples, 17.24% samples were positive for M. muris by PCR method, while 35.93% cultures showed positive.The phylogenetic analysis indicated a new strain of M. muris.The results of culture and PCR methods displayed the contamination in NIH mice.Therefore, Therefore, more researches are needed regarding the presence of mycoplasma for treatment and clinical signs.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".