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Record W2782320948 · doi:10.1016/j.stemcr.2017.12.008

Transplanted Donor- or Stem Cell-Derived Cone Photoreceptors Can Both Integrate and Undergo Material Transfer in an Environment-Dependent Manner

2018· article· en· W2782320948 on OpenAlexfundno aff
Paul V. Waldron, Fabiana Di Marco, Kamil Kruczek, Joana Ribeiro, Anna B. Graca, Claire Hippert, Nozie D. Aghaizu, Aikaterini A. Kalargyrou, Amanda C. Barber, Giulia Grimaldi, Yanaí Durán, Samuel J.I. Blackford, Magdalena Kloc, Debbie Goh, Eduardo Zabala Aldunate, Robert D. Sampson, James Bainbridge, Alexander J. Smith, Anai Gonzalez-Cordero, Jane C. Sowden, Robin R. Ali, R. A. Pearson

Bibliographic record

VenueStem Cell Reports · 2018
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRetinal Development and Disorders
Canadian institutionsnot available
FundersH2020 European Research CouncilEuropean Research CouncilRetina UKMedical Research CouncilRoyal SocietyFight for Sight UKFoundation Fighting BlindnessNational Institute for Health and Care ResearchAlcon Research InstituteNIHR Bristol Biomedical Research CentreMoorfields Eye CharityGreat Ormond Street Hospital for ChildrenWellcome TrustMacula Vision Research Foundation
KeywordsBiologyStem cellCone (formal languages)Cell biologyTransplantationInternal medicine

Abstract

fetched live from OpenAlex

Human vision relies heavily upon cone photoreceptors, and their loss results in permanent visual impairment. Transplantation of healthy photoreceptors can restore visual function in models of inherited blindness, a process previously understood to arise by donor cell integration within the host retina. However, we and others recently demonstrated that donor rod photoreceptors engage in material transfer with host photoreceptors, leading to the host cells acquiring proteins otherwise expressed only by donor cells. We sought to determine whether stem cell- and donor-derived cones undergo integration and/or material transfer. We find that material transfer accounts for a significant proportion of rescued cells following cone transplantation into non-degenerative hosts. Strikingly, however, substantial numbers of cones integrated into the Nrl −/− and Prph2 rd2/rd2 , but not Nrl −/− ; RPE65 R91W/R91W , murine models of retinal degeneration. This confirms the occurrence of photoreceptor integration in certain models of retinal degeneration and demonstrates the importance of the host environment in determining transplantation outcome.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.198
Teacher spread0.190 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations131
Published2018
Admission routes1
Has abstractyes

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Same venueStem Cell ReportsSame topicRetinal Development and DisordersFrench-language works237,207