A Genomic Screen of Saccharomyces cerevisiae Reveals a Potential Link Between Nonstop Decay and Autophagy
Bibliographic record
Abstract
Little has been described about the mechanisms of the Nonstop Decay (NSD) pathway.To discover new genes involved in NSD, we performed a genome wide investigation in Saccharomyces cerevisiae which yielded 446 gene candidates that when deleted, allowed production of a nonstop mRNA.Of the gene families found in our screen, we decided to follow up on the autophagy candidates.First, we used the autophagy inhibiting drug verteporfin to confirm that autophagy does not occur in our candidates.The results confirm our prediction that autophagy is inhibited in our two autophagy candidates.Next, we assessed the abundance of nonstop mRNA using qRT-PCR analysis.This resulted in lower than expected nonstop mRNA abundance relative to the WT, which led us to investigate nonstop protein abundance.Interestingly, we see an increase in the abundance of nonstop protein abundance relative to the WT.Together, our results suggest that NSD may occur through autophagy.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".