First report of a ‘<i>Candidatus</i> Phytoplasma asteris‘‐related strain (16SrI‐B) associated with <i>Sonchus oleraceus</i> (common sowthistle) phyllody disease in Iran
Bibliographic record
Abstract
Common sowthistle (Sonchus oleraceus) is a major weed problem in Iran and is also used for medicinal purposes. During 2012-2015 surveys of Fars and Yazd provinces (Iran) for phytoplasma diseases, a phyllody disease was observed in S. oleraceus plants growing as weeds within and/or in the surroundings of fields and orchards. The main symptoms of the disease were yellowing and reddening of leaves, shortened internodes, flower virescence, phyllody, proliferation and witches' broom (Fig. 1). Total DNA was extracted from 0.2 g of leaf midribs of eight symptomatic (four plants per province) and four symptomless (collected in Fars province) S. oleraceus plants using the CTAB method of Zhang et al. (6). DNA samples were tested for phytoplasma presence by a nested PCR assay primed by primer pairs P1/P7 followed by R16F2n/R16R2 (Lee et al., 3). Amplicons of ~1.25 kb were obtained in the nested round from all eight diseased plants but not from the four symptomless plants. Eight nested amplicons were separately cloned and sequenced. The obtained sequences were identical and a consensus sequence corresponding to the Abarkooh (Yazd Province, Iran) S. oleraceus phyllody phytoplasma was deposited in GenBank (Accession No. MG652627). The S. oleraceus phyllody phytoplasma strain had 99.78% sequence identity with the ‘Ca. P. asteris’ reference strain (M30790). A BLAST search showed that this sequence had maximum identity (99-100%) with members of the 16SrI subgroup B. Phylogenetic analysis using the neighbour-joining method (MEGA7) (Fig. 2) showed that the Abarkooh S. oleraceus phyllody phytoplasma was clustered within the 16SrI group closest to onion yellows phytoplasma (NC_005303), representative of subgroup 16SrI-B. A 16SrI-A phytoplasma has been previously reported in S. oleraceus in Canada (Khadhair et al., 2), however to our knowledge this is the first report of a 16SrI-B phytoplasma associated with S. oleraceus phyllody disease. The occurrence of 16SrI-B phytoplasma strains were previously reported in Iran on Brassica napus (Salehi et al., 5), Eruca sativa (Esmailzadeh Hosseini et al., 1) and Eucalyptus camaldulensis (Salehi et al., 4); S. oleraceus may act as a reservoir host.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".