A212 EXPRESSION AND CHARACTERIZATION OF SOLUBLE PEKIN DUCK PROGRAMMED DEATH-1
Bibliographic record
Abstract
Programmed death 1 (PD-1, CD279) negatively regulates TCR complex-initiated signaling by interacting with its cognate ligands PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD 273). Recently, therapeutic targeting aimed at abrogation of co-inhibition through PD-1 has led to breakthroughs in the treatment of cancer and has shown potential for the treatment of chronic viral infections. We have identified the duck PD-1, PD-L1 and PD-L2 proteins that have a sequence identity of 31%, 45% and 36% with human PD-1, PD-L1 and PD-L2, respectively. The aim of this study was to express soluble duck PD-1 (duPD-1) for functional characterization in the duck hepatitis B model. The duPD-1 ectodomain (501 bp) was inserted into pZeo2B in frame with a polyHis tag and expressed in 293T cells. Culture supernatants were assessed immunoblot and soluble duPD-1 was affinity purified and conjugated with RPE. Full-length PD-L1 and PD-L2 fused in frame with eGFP were inserted in pZeo2B and expressed in 293T cells, respectively. Binding of soluble duPD-1 to duPD-L1 and duPD-L2 were assessed by FACS in PD-L1-eGFP-, PD-L2-eGFP- and eGFP-transfected 293T cells. Soluble duPD-1 could be expressed as a secreted protein in 293T cells. Affinity purified soluble duPD-1 conjugated with RPE could bind to duPD-L1 and duPD-L2 expressed on 293T cells. Soluble duPD-1 can be expressed as a secreted protein and retains binding affinity to duPD-L1 and duPD-L2 that can be used to study the role of PD-1 co-inhibition in the duck hepatitis B infection model. CIHRCanadian Liver Foundation (CLF) and the Canadian Foundation for Innovation (CFI).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".