A296 THE ANTI-COLITIC EFFECT OF INFECTION WITH HYMENOLEPIS DIMINUTAIS NOT OBSERVED IN ANTIBIOTIC TREATED MICE.
Bibliographic record
Abstract
Infection with helminth parasites can ameliorate concomitant inflammatory disease: we showed that mice infected with H. diminuta display significantly less sever di-nitrobenzene sulphonic acid (DNBS)- induced colitis. The commensal bacteria make a significant contribution to gut homeostasis and perturbation of this complex microbiota is often observed in colitis. Thus, the hypothesis arises that H. diminuta evoked protection against colitis is mediated, in least in part, via changes in the colonic microbiota. To determine if infection with the helminth parasite, H. diminuta, evokes distinct changes in the colonic microbiota that are required for the anti-inflammatory effect in the DNBS model of colitis. Bacterial DNA was isolated from colon tissue samples from control and H. diminuta-infected (5 cysticercoids) Balb/c ± DNBS treatment (3 mg, ir, 72h) and amplified using 16s rRNA primers prior to sequencing (Illumina miSeq). Sequence results were demultiplexed and OTU’s were generated on the University of Calgary’s MetaAmp pipeline and mice. Colitis was assessed by colon length, weight loss, a cumulative disease activity score and histopathology. The anti-colitic effect of infection with H. diminuta was assessed in mice given a broad spectrum antibiotic (Abx) regimen. Sequence analysis of the microbiota of H. diminuta-infected and control mice revealed no statistical differences. Abx-treated mice produced a natural Th2 and anti-helminth immune response towards infection with H. diminuta. H. diminuta-infected mice were protected from colitis. DNBS-treated mice ± H. diminuta had a similar colonic microbiota with a major shift from firmicutes to bacteroidetes/proteobacteria. However, H. diminuta-evoked protection against DNBS-induced colitis was significantly reduced in mice with a colonic microbiota that had been significantly reduced by antibiotic treatment (n=4). Infection with H. diminuta may have subtle effects on the colonic microbiota of mice, but the bacteria appear not to be a requirement for either the induction of a helminth-directed TH2 response or successful expulsion of H. diminuta. While there were no striking differences in the microbiota of DNBS versus DNBS+H. diminuta treated mice, the anti-colitic effect of infection with H. diminuta was negated in antibiotic-treated mice. Suggesting that bacteria are involved in this beneficial effect of infection with a cestode parasite in a non-permissive system. NSERC, NSERC CREATE Host-Parasite, and Beverly Phillips Scholarship (University of Calgary)
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.007 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".