A8 INFLUENCE OF MATERNAL SSRI EXPOSURE ON THE DEVELOPMENT OF THE ENTERIC NERVOUS SYSTEM
Bibliographic record
Abstract
Antidepressants, including selective serotonin reuptake inhibitors (SSRIs) are commonly used during pregnancy. Approximately 7% of women in North America require SSRI in the perinatal period. Perinatal exposure to SSRIs has been shown to disrupt the development of serotonergic signaling pathways in the brain. However, the potential for changes to the enteric nervous system (ENS) over a period of postnatal development has not been explored. To test the hypothesis that perinatal exposure to SSRIs can influence the development of the ENS. Female Wistar rats were given fluoxetine (10mg/kg/d) or vehicle (cookie dough) from 2 weeks prior to mating until weaning (postnatal day [P] 21). Offspring were collected from SSRI-treated and control rats on P1, P21 and P6 months to see if changes were persistent. Enteric neurons in the myenteric plexus were visualized in whole mount preparations of jejunum, ileum and colon. Total number (#) of enteric neurons (EN) and serotonergic neurons (SN) were visualized with immunostaining using antibodies to HuC/D and 5-HT, respectively. Percentage of SSRI-expressing enteric neurons were calculated as a percentage of total neurons using image analysis software (Velocity). Female (F) and male (M) offspring were analyzed separately. On P1, no significant differences were found in the total # of EN nor in SN between SSRI-exposed and control offspring in the jejunum, ileum and M colon (n=6–10). However, a significant decrease between the total # of EN and SN was found in the SSRI-exposed F offspring colon (13.6% vs 9.3%; p=0.04; n=6–9). On P21, no significant differences were found in the total # of EN in the ileum and colon of SSRI-exposed and control offsprings. There was also no significant difference found between the percentage of SN between the SSRI-exposed and control ileum (n=6–10). Significant differences were found in the percentage of SN in both F (1.4% vs 7.6%; p=0.009); n=5–6) and M colons (1.7% vs 6.9%; p=0.002; n=5–8). At 6 months of age, there was no significant difference in the percentage of total # of EN nor in SN between SSRI-exposed and control offspring. Our results suggest that SSRI exposure in the in utero and perinatal period play a role in serotonergic signaling pathways involved in the development of the enteric nervous system of the developing offspring. However, these changes improve over time, which may be attributed to the plasticity of the ENS. CCFA Broad Foundation
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".