Rac1b, A Variant Of Rac1 Interacts With Calmodulin
Bibliographic record
Abstract
Rac1b, a splice isoform of Rac1, is up‐regulated in malignant colorectal and breast cancer. It contains an additional 19 amino acid insertion close to the switch II domain, a region important for Rac1 interaction with regulators and effectors. This insertion leads to decreased GTPase activity and reduced affinity for GDP, resulting in intracellular predominance of GTP‐bound Rac1b. Previously, a 14 amino acid region essential for calmodulin (CaM) binding has been established in Rac1. A similar region exists in Rac1b. This led to our hypothesis that Rac1b interacts with CaM. Western Blot analysis showed that endogenous Rac1b interacts with CaM‐Sepharose, and that Rac1b binds to pure CaM, in a calcium dependent manner. Experiments using W7, a potent CaM antagonist, revealed that W7 does not play a role in GDP/GTP binding to Rac1b. However, the role of CaM in the activation and function of Rac1b still remains to be analyzed and is being pursued.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".