IpdAB, a virulence factor in <i>Mycobacterium tuberculosis</i> , is a cholesterol ring-cleaving hydrolase
Bibliographic record
Abstract
Significance All steroid-degrading bacteria utilize IpdAB, a predicted CoA transferase (CoT) that has been implicated in the hydrolysis of a carbon–carbon bond, an unprecedented reaction in CoTs. In Mycobacterium tuberculosis , IpdAB is required for degrading host cholesterol and virulence. We used a combination of X-ray crystallographic and biochemical studies to elucidate the mechanism of IpdAB. Superposition of the IpdAB Mtb active site with those of CoTs reveals distinct architectural features which, in conjunction with the biochemical data, indicate that IpdAB catalyzes a retro-Claisen-like ring-opening reaction. This reaction is unique for a member of the CoT superfamily. This study provides insights into bacterial steroid catabolism and facilitates the development of potential antituberculosis therapeutics targeting IpdAB.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".