Bibliographic record
Abstract
Perfluoroalkyl acids (PFAAs) are a family of emerging toxicants that are non-biodegradable, and have the potential to bioconcentrate and biomagnify. In an attempt to answer questions about human exposure to PFAAs, and subsequent human disposition of these compounds which have public health implications, four hypothesis-driven and multidisciplinary research projects were conducted. The first project used an isomer-specific LC-MS/MS method to characterize the PFAA isomer signatures in house dust, and whether PFAA isomers could cross the human placenta to different extents. The findings showed that a Canadian population was currently exposed to a mix of both telomer and electrochemical perfluorooctanoate (PFOA), despite the latter having been phased out since 2002. Strong evidence for the preferential transfer of branched perfluorooctanesulfonate (PFOS) and PFOA isomers, compared to linear, was found by comparing 20-paired maternal and cord blood samples. The second project was conducted on a local family of seven individuals, all of whom had exceptionally high serum levels of perfluorohexanesulfonate (PFHxS). A forensic investigation was conducted in the family’s dwelling, and data revealed that the source of the high exposure in this family was the house carpets which had been treated multiple times with Scotchgard™ formulations over a period of 15 years. The pathway of exposure was most-likely through inhalation and ingestion of house dust. A series of in vitro experiments were conducted in a third project to assess the dissociation constants (Kd) of linear and branched PFOA and PFOA isomers. Kd’s of the linear isomers were lower than the branched isomers, and in technical mixtures spiked to serum it was evident that the branched isomers were less bound to serum protein, and thus would be more bioavailable to cross into fetal circulation or be excreted by the kidney. The last research project modeled the transplacental transfer of PFAAs in-vitro using cultured human placental syncytiotrophoblasts. This model confirmed the findings of the first study, to the effect that branched isomers, especially in the case of PFOA, crossed the placental barrier more efficiently than linear molecules, independent of their serum protein binding. Overall, this thesis contributed unique observations from biomonitoring of PFAAs at the isomer-specific level, and follow-up experiments helped to explain these while furthermore contributing to our understanding of how PFAAs are biopersistent in humans.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".