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Record W27984013 · doi:10.1016/j.ab.2016.10.022

Testis-specific Protein Kinases TSSK1 and TSSK2 in Mouse Spermiogenesis

2014· article· en· W27984013 on OpenAlexfundno aff
Peng Shang

Bibliographic record

VenueAnalytical Biochemistry · 2014
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Canadian institutionsnot available
FundersNatural Sciences and Engineering Research Council of CanadaNational Natural Science Foundation of China
KeywordsBiologySpermatidSpermiogenesisKinaseCell biologyGeneGeneticsSperm

Abstract

fetched live from OpenAlex

__Abstract__ \n \nIn this thesis, author systematically studied two testis-specific protein kinases \nTSSK1 and TSSK2 in mouse spermiogenesis. By building a double knockout \nmouse model with the absence of Tssk1 and Tssk2 genes, it was demonstrated \nthat TSSK1 and TSSK2 are essential for male fertility in mouse, and the homozygous \nmale mutants missing these two kinases are infertile. In addition, TSSK1 and \nTSSK2, together with their common substrate, TSKS, are involved into the \nchromatoid body (CB) transformation during spermatid elongation. By co-IP and \nfollowing proteomics analyses, it was shown that TSSK1, TSSK2, and TSKS can form a stable protein complex, named as TSSK1/2-TSKS complex, which is \ninvolved into RNA regulation, protein phorsphorylation and cytoskeleton regulation. \nFurther evolutionary studies displayed that TSSK1 and TSSK2 gained diverse \nfunctions during evolution, although Tssk1 and Tssk2 are a pair of duplicated \ngenes, and TSSK1 and TSSK2 are highly homologous. Because of the \ntestis-specificty, late expression, and the indispensability to male fertility, TSSK1 \nand TSSK2 are potential drug targets for non-hormonal contraceptives

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.246
Teacher spread0.235 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2014
Admission routes1
Has abstractyes

Explore more

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