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Record W2800781184 · doi:10.1002/cbin.10977

Myricetin‐induced oxidative stress suppresses murine T lymphocyte activation

2018· article· en· W2800781184 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueCell Biology International · 2018
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics, phytochemicals, and oxidative stress
Canadian institutionsDalhousie University
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsMyricetinOxidative stressT cellChemistryCytotoxic T cellImmune systemReactive oxygen speciesAntigen-presenting cellMolecular biologyCell biologyBiologyBiochemistryAntioxidantImmunologyFlavonoidIn vitroKaempferol

Abstract

fetched live from OpenAlex

Abstract A number of polyphenolic compounds present in fruits and vegetables have the capacity to modulate immune responses; however, the impact of the common plant‐derived flavonoid myricetin on T lymphocyte function has not been investigated. We show that myricetin inhibited mouse T lymphocyte activation by bead‐immobilized anti‐CD3 and anti‐CD28 monoclonal antibodies, as indicated by a dose‐dependent reduction in cell proliferation and decreased synthesis of interferon‐γ, interleukin (IL)‐2, IL‐4, and IL‐17 associated with different T helper cell subsets. This effect was attributed to myricetin‐induced reactive oxygen species (ROS) since myricetin caused hydrogen peroxide (H 2 O 2 ) to accumulate in cell‐free culture medium and H 2 O 2 inhibited T cell proliferation and cytokine synthesis. In addition, the antioxidant N‐acetyl cysteine restored the ability of myricetin‐treated T lymphocytes to proliferate in response to a mitogenic stimulus. The presence of dendritic cells or bone marrow‐derived macrophages negated the inhibitory effect of myricetin on T cell activation, and H 2 O 2 in T cell cultures that were treated with exogenous H 2 O 2 was reduced when antigen‐presenting cells were also present. These findings suggest that antioxidant molecules produced by dendritic cells and macrophages protected T cells from myricetin‐induced oxidative stress, and underscore the importance of considering immune cell interactions when evaluating the immunomodulatory activity of ROS‐generating phytochemicals.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.032
Threshold uncertainty score0.771

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.267
Teacher spread0.256 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it