Abstract 16872: Increased Production of Oxidized Phosphatidylcholine Molecules in Patients Presenting With ST Elevation Myocardial Infarction (STEMI)
Bibliographic record
Abstract
Introduction: Despite restoration of blood flow to myocardium after ischemia, a large percentage of myocardial cells die after reperfusion. This phenomenon is termed ischemia/reperfusion injury (I/R). Oxidized phosphatidylcholines (OxPC) are generated in the presence of reactive oxygen species, which are abundant during I/R. We aimed to determine the change in OxPC in patients presenting with STEMI who undergo primary Percutaneous Coronary Intervention (PCI). Methods: Blood samples were collected from subjects with STEMI (n=27) and subjects with non-obstructive CAD (n=20) prior to, and at completion of PCI/angiography respectively. The complete oxidative lipidomic profile at the onset of STEMI and after PCI was compared to control prior to and after diagnostic angiography. The oxidative lipidomic profile was established and compared using high-performance electrospray mass spectrometry. Results: Seventy nine distinct OxPCs were identified in human plasma which included fragmented OxPCs, terminal furans, isoprostanes and long-chain products. There were 17 compounds that showed a significant increase in the STEMI population when compared to control. The largest changes seen overall were in the fragmented OxPCs. Fragmented OxPCs in control vs pre PCI STEMI had a significant rise from 8.9 vs 21.8 ng/ml of plasma, (p = 0.0001) and STEMI post PCI increased to 8.9 vs 29.7 ng/ml of plasma, (p = 7.1x10-5). When organizing molecules by functional group, fragmented carboxylic acid OxPCs were the most abundant in plasma with pre vs post PCI levels increasing from 12.8 to 22.2 ng/ml of plasma (p= 0.03). Conclusion: We have shown for the first time that OxPC molecules are present in patients with STEMI and are increased when compared to control patients. There is also a significant increase in OxPC levels after PCI in the STEMI population. These changes represent increases in OxPC during ischemia and a further increase after reperfusion injury. Given the potent biological activity of OxPCs, the attenuation of their activity may be a novel therapeutic approach to reduce the deleterious effects of I/R.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".