Molecular cloning and induced expression of the plasma membrane intrinsic protein gene and promoter from mulberry (<i>Morus multicaulis</i>)
Bibliographic record
Abstract
To isolate the plasma membrane intrinsic protein 2 (PIP2) gene and its promoter from mulberry (Morus multicaulis), and to analyse its expression under stress conditions using quantitative real-time polymerase chain reaction (qRT-PCR) analysis, the mulberry MmPIP2 gene was cloned and sequenced. The putative MmPIP2 protein was 282 amino acids long and contained Asn–Pro–Ala signature motifs. Phylogenetic analysis showed that MmPIP2 is highly conserved, exhibiting strong homology with other plant PIP2s. The 5′ flanking sequence was cloned by genome walking and numerous transcription factor binding sites were identified. qRT-PCR revealed that the expression levels of MmPIP2 differed with changes in abiotic stress, indicating that PIP2 is a multifunctional molecule in mulberry. Studying the molecular mechanisms behind adaptations to stress and strengthening stress tolerance in plants are of fundamental importance to mulberry production.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".