Liposome-based therapy: An alternative approach to treat Helicobacter pylori infection in high prevalence communities such as Aklavik, NWT
Bibliographic record
Abstract
Helicobacter pylori infects about half of the world population causing chronic gastritis, peptic ulcer or gastric cancer. The Aboriginal people in Aklavik, NWT, Canada are concerned about gastric cancer because of the high prevalence of H. pylori infection (58%) in their community. H. pylori colonization of the acidic stomach environment is dependent on the production of urease enzyme and bacterial adhesins such as BabA. Toxins produced by H. pylori, CagA and VacA, contribute to the development of stomach diseases. Standard therapies often fail due to antimicrobial resistance and lack of compliance. Liposomes are lipid vehicles that have wide therapeutic applications in enhancing the delivery of drugs and genes into different cells. This thesis characterizes H. pylori isolated from residents of Aklavik for major virulence genes, describes the prevalence of gastric biopsy histopathology outcomes, and estimates the effect of H. pylori virulence genotype on the prevalence of gastric biopsy histopathology outcomes. In addition, liposome-based treatment approaches are investigated as alternative treatment approaches for H. pylori eradication. This thesis is the first report on the frequencies of H. pylori genotype and histopathology outcomes for the community of Aklavik, NWT, and identifies the association of virulence genes cagA and vacA subtypes with gastritis and/or intestinal metaplasia. Cationic liposome-enhanced delivery of urease antisense DNA inhibited urease expression by 40%, which may interfere with H. pylori survival in acidic conditions. Alterations in liposome composition to improve liposome stability did not improve delivery of antisense DNA to H. pylori. However, cationic liposomes themselves were previously reported to have antimicrobial activity against some bacteria and protozoans at concentrations that are nontoxic to mammalian cells. This thesis is the first demonstration of the enhanced effect of liposomes on the delivery of urease antisense DNA to H. pylori resulting in impaired urease expression and activity, and also the first report on the antimicrobial activity of stearylamine-containing cationic liposomes against H. pylori. The novel findings presented in this thesis provide the potential for alternative treatment approaches for the eradication of H. pylori infections, important strategies for communities where there is a high prevalence of H. pylori infection.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".