Effects of sodium bicarbonate on cell growth, lipid accumulation, and morphology of Chlorella vulgaris
Bibliographic record
Abstract
Low concentration NaHCO 3 (ca. 12 mM) had been demonstrated to be an excellent carbon source for industrially important green alga Chlorella vulgaris and high concentration NaHCO 3 (e.g. 160 mM) had been shown to be capable of controlling protozoa and stimulating lipid accumulation of another green alga, i.e., Neochloris oleoabundans . Furthermore, little was known about the mechanisms of the effects of NaHCO 3 on microalgae. Thorough studies on the effects of high NaHCO 3 on C. vulgaris and their mechanisms were therefore warranted. We systematically compared the cell growth, lipid production, and cell morphology of the industrially important C. vulgaris in 160 mM NaHCO 3 or 160 mM NaCl media at different pH levels. These data allowed us to analyze the effects of total dissolved inorganic carbon (DIC) and individual DIC species on C. vulgaris . Cell growth of C. vulgaris at a range of concentrations at 160 mM or lower was also studied. Cellular lipid cell content of 494 mg g −1 and lipid productivity of 44.5 mg L −1 day −1 were obtained at 160 mM NaHCO 3 and pH 9.5. High concentration NaHCO 3 (e.g. 160 mM) was inhibitive to cell growth but stimulating to lipid accumulation and caused unicellular C. vulgaris to transfer to colonial cells. Increasing pH in the range of 7.5–9.5 caused increasing inhibition to cell growth in 160 mM NaCl. Whereas the optimal pH for cell growth was 8.5 for 160 mM NaHCO 3 cultures. Comparative experiments with 0–160 mM NaHCO 3 indicate that 10 mM was the optimal concentration and increasing NaHCO 3 from 10 to 160 mM caused increasing inhibition to cell growth. High concentration DIC was inhibitor to cell growth but stimulator to lipid accumulation of C. vulgaris . It caused unicellular C. vulgaris to transform to colonial cells. Results suggest that high concentration of a particular DIC species, i.e., dCO 2 , was the primary stress responsible for cell growth inhibition. Where CO 3 2− was likely the DIC species responsible for lipid stimulation of C. vulgaris . Furthermore, we propose that the colony formation at high DIC conditions was employed by C. vulgaris to mitigate the stress by minimizing cell exposure to unfavorable environment.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".