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Record W283545491

Methods of Protein Characterization

2011· article· en· W283545491 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

venuePublished in a venue whose home country is Canada.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueSound Ideas (University of Puget Sound) · 2011
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMicrobial Metabolic Engineering and Bioproduction
Canadian institutionsnot available
Fundersnot available
KeywordsCharacterization (materials science)Computer scienceNanotechnologyMaterials science
DOInot available

Abstract

fetched live from OpenAlex

AppA is a protein in Rhodobacter sphaeroides that has been the topic of debate among scientists for the past several years with regards to the structure of the protein. It has been known that AppA has an effect on the activity of PpsR, which controls the gene expression of photosystems. There are two conflicting experimental structures (2IYG and 1YRX) of the protein, both of which claim to be taken in the dark phase (meaning when there is no light shining on the protein). The debate is about whether some slight differences in the structures represent the shift from the dark state to the light-induced state. It is known that in the light state, AppA releases PpsR and undergoes a dimerization event with itself. We observed the interactions within an experimental structure of the AppA dimer and compared them to the 2IYG and 1YRX structures. Most scientists have focused on the positioning of Trp104 to explain the functional changes of AppA upon light excitation, but our analysis of experimental structures suggests that the dimerization could play a larger role in the functional mechanism of AppA.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.372
Threshold uncertainty score0.367

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.225
Teacher spread0.209 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it