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Record W2883374764 · doi:10.1055/s-0038-1644920

Inhibition of Cellular Senescence in AD Models with Water Soluble CoQ10 via Upregulation in Autophagy

2018· article· en· W2883374764 on OpenAlex
Simon Pupulin, Caleb Vegh, Lauren Culmone, Rachel Huggard, Iva Okaj, Darcy Wear, Siyaram Pandey

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenuePlanta Medica International Open · 2018
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCoenzyme Q10 studies and effects
Canadian institutionsUniversity of Windsor
Fundersnot available
KeywordsAutophagyOxidative stressSenescenceCell biologyProgrammed cell deathCoenzyme Q10Reactive oxygen speciesDownregulation and upregulationMitochondrionBiologyPopulationWestern blotHippocampusAlzheimer's diseaseApoptosisChemistryNeuroscienceMedicineDiseasePathologyBiochemistryGene

Abstract

fetched live from OpenAlex

Alzheimer's disease (AD) is a progressive neurodegenerative disorder often associated with memory impairment. According to the World Health Organization, approximately 48 million people worldwide live with the disease and this number is expected to triple by 2050. Due to the increasing prevalence of AD and the rapidly aging global population, a preventative treatment is absolutely necessary. AD is a complex and poorly understood disease with no current treatments to stop its progression. Still, some characteristics of the disease are known including the formation of neurofibrillary tangles and amyloid plaques which have been linked to neuronal death in regions of the hippocampus and cerebral cortex. Additionally, studies have shown a number of mechanisms associated with the disease including impaired autophagy and mitochondrial dysfunction leading to oxidative stress. Elevated reactive oxygen species are a result of inefficiency in the electron transport chain and can induce premature cellular senescence. However, in previous in-vitro studies, ubisol-Q10, a water-soluble formulation of coenzyme-Q10, has been shown to stabilize the mitochondria and inhibit oxidative-stress by reducing the generation of free radicals. When this treatment was applied to a fibroblast cell model of AD and gene expression was analyzed and an increase in autophagy related gene expression was noted. This proposal focuses on determining if autophagy is activated by ubisol-Q10 during stressed induced cellular senescence by examining relative protein expression levels via Western Blot. This will be coupled with immunohistochemistry on an animal tissue model and live cell staining to confirm the role of autophagy as a pro-survival response.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.027
Threshold uncertainty score0.247

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.249
Teacher spread0.238 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it