Non-polar solvent fractions of Oplopanax horridus stimulate muscle glucose uptake and inhibit hepatocellular glucose-6-phosphatase enzyme activity
Bibliographic record
Abstract
See also: Non-polar solvent fractions of Oplopanax horridus stimulate muscle glucose uptake and inhibit hepatocellular glucose-6-phosphatase enzyme activity Planta Medica International Open 2018; 5(S 01): DM03 DOI: 10.1055/a-0819-0090 Canadian First Nation (FN) populations exhibit a higher prevalence of obesity and Type 2 diabetes than non-Indigenous Canadians. Moreover, modern treatments are not culturally well adapted and several FN wish to explore the benefits of their Traditional Medicine. British Columbia FN (notably, Squamish) use Oplopanax horridus (OH) or Devil's club for health benefits, including for diabetes. We studied the antidiabetic potential of OH on muscle glucose transport and hepatocellular glucose homeostasis. Methodology: A classical bioassay-guided phytochemical fractionation approach was used to identify potential solvent fractions responsible of the antidiabetic actions of OH. Differentiated C2C12 myocytes were treated with the following OH preparations: hot water (HWE) and ethanol (EtOH) crude extracts (12.5 µg/ml); specific solvent fractions: hexanes (HEX; 1.12 µg/ml); dichloromethane (DCM), ethyl acetate (EtOAc), methanol (MeOH), water (H2O) as well as chlorogenic acid (CA) (all 12.5 µg/ml). Radiolabelled glucose uptake was measured. H4IIE (rat hepatoma cells) were treated with the same OH preparations and glucose-6-phosphatase (G6Pase) activity measured. Results: EtOH extract, as well as HEX, DCM, EtOAc fractions significantly stimulated muscle glucose uptake by 130 – 204%, compared to DMSO control (set at 100%). In contrast, only DCM fraction significantly inhibited hepatocellular G6Pase activity (24%). Other preparations mildly reduced G6Pase (12 – 17%). Conclusion: These results suggest an antidiabetic potential of Oplopanax horridus in liver and skeletal muscle cells in culture. The phytochemical fractionation approach will be pursued further to identify specific compounds contained in OH preparations that contribute to its antidiabetic potential. Please note: this abstract was changed according to the following erratum: the name of the second author was changed.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".