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Record W2885309985 · doi:10.1073/pnas.1806239115

Nitro-fatty acids are formed in response to virus infection and are potent inhibitors of STING palmitoylation and signaling

2018· article· en· W2885309985 on OpenAlexaff
Anne Louise Hansen, Gregory J. Buchan, Michael Rühl, Kojiro Mukai, Sonia R. Salvatore, Emari Ogawa, Sidsel Dahl Andersen, Marie B. Iversen, Anne L. Thielke, Camilla Gunderstofte, Mona Motwani, Charlotte Møller, Andreas S. Jakobsen, Katherine A. Fitzgerald, Jessica Roos, Rongtuan Lin, Thorsten J. Maier, Raphaela Goldbach‐Mansky, Cathrine A. Miner, Wei Qian, Jonathan J. Miner, Rachel E. Rigby, Jan Rehwinkel, Martin R. Jakobsen, Hiroyuki Arai, Tomohiko Taguchi, Francisco J. Schöpfer, David Olagnier, Christian K. Holm

Bibliographic record

VenueProceedings of the National Academy of Sciences · 2018
Typearticle
Languageen
FieldImmunology and Microbiology
Topicinterferon and immune responses
Canadian institutionsMcGill University
FundersCore Research for Evolutional Science and TechnologyNational Institute of Diabetes and Digestive and Kidney DiseasesJapan Society for the Promotion of ScienceOno Medical Research FoundationNational Institutes of HealthH. Lundbeck A/SNational Institute of General Medical SciencesAugustinus FondenLundbeckfondenJapan Agency for Medical Research and DevelopmentMedical Research CouncilAarhus UniversitetAmerican Heart Association
KeywordsStingStimulator of interferon genesPalmitoylationBiologyTANK-binding kinase 1InterferonSignal transductionImmunologyCell biologyBiochemistryCytosolEnzymeCysteine

Abstract

fetched live from OpenAlex

The adaptor molecule stimulator of IFN genes (STING) is central to production of type I IFNs in response to infection with DNA viruses and to presence of host DNA in the cytosol. Excessive release of type I IFNs through STING-dependent mechanisms has emerged as a central driver of several interferonopathies, including systemic lupus erythematosus (SLE), Aicardi-Goutières syndrome (AGS), and stimulator of IFN genes-associated vasculopathy with onset in infancy (SAVI). The involvement of STING in these diseases points to an unmet need for the development of agents that inhibit STING signaling. Here, we report that endogenously formed nitro-fatty acids can covalently modify STING by nitro-alkylation. These nitro-alkylations inhibit STING palmitoylation, STING signaling, and subsequently, the release of type I IFN in both human and murine cells. Furthermore, treatment with nitro-fatty acids was sufficient to inhibit production of type I IFN in fibroblasts derived from SAVI patients with a gain-of-function mutation in STING. In conclusion, we have identified nitro-fatty acids as endogenously formed inhibitors of STING signaling and propose for these lipids to be considered in the treatment of STING-dependent inflammatory diseases.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.031
GPT teacher head0.294
Teacher spread0.263 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations292
Published2018
Admission routes1
Has abstractyes

Explore more

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