The Arabidopsis Mitogen-Activated Protein Kinase Kinase Kinase 20 (MKKK20) C-terminal domain interacts with MKK3 and harbors a typical DEF mammalian MAP kinase docking site
Bibliographic record
Abstract
Mitogen-activated protein kinase (MAPKs) constitute a major component in plant cellular signaling considering the sheer number of MAPKKKKs, MAPKKKs, MAPKKs and MAPKs when compared to yeast and animal systems. Nevertheless, only few complete MAPK cascades have been deciphered and the same hold true for their substrates. Furthermore, cascades often share kinase components, but little is known about their specific interactions and domains. The Arabidopsis thaliana MAP kinase kinase kinase 20 (MKKK20) was recently showed to interact with MKK3 and MPK18 in two non-complementary signaling cascades involved in root cortical microtubule functions. Here, MKKK20 and MKK3 proteins where dissected and tested in yeast two-hybrid assays followed by an in planta validation through bimolecular fluorescence complementation (BiFC) assays and showed that the MKKK20 C-terminal region interacted with MKK3 that comprised a typical DEF domain akin to MAPKs docking domains.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".