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Record W2885780647 · doi:10.1101/371450

Genetic discovery and translational decision support from exome sequencing of 20,791 type 2 diabetes cases and 24,440 controls from five ancestries

2018· preprint· en· W2885780647 on OpenAlexaff
Jason Flannick, Josep M. Mercader, Christian Fuchsberger, Miriam S. Udler, Anubha Mahajan, Jennifer Wessel, Tanya M. Teslovich, Lizz Caulkins, Ryan Koesterer, Thomas W. Blackwell, Eric Boerwinkle, Jennifer A. Brody, Ling Chen, Siying Chen, Cecilia Contreras-Cubas, Emilio J. Córdova, Adolfo Correa, Maria L. Cortés, Ralph A. DeFronzo, Lawrence M. Dolan, Kimberly L. Drews, Amanda Elliott, James S. Floyd, Stacey Gabriel, María Eugenia Garay-Sevilla, Humberto Garcia‐Ortíz, Myron D. Gross, Sohee Han, Sarah C. Hanks, Nancy L. Heard‐Costa, Anne Jackson, Marit E. Jørgensen, Hyun Min Kang, Megan M. Kelsey, Bong-Jo Kim, Heikki A. Koistinen, Johanna Kuusisto, Joseph B. Leader, Allan Linneberg, Ching‐Ti Liu, Jianjun Liu, Valeriya Lyssenko, Alisa K. Manning, Anthony Marcketta, Juan Manuel Malacara-Hernández, Angélica Martínez‐Hernández, Karen Matsuo, Elizabeth J. Mayer‐Davis, Elvia Mendoza‐Caamal, Karen L. Mohlke, Alanna C. Morrison, Anne Ndungu, Maggie C. Y. Ng, Colm O’Dushlaine, A. J. Payne, Catherine Pihoker, Wendy S. Post, Michael Preuß, Bruce M. Psaty, Ramachandran S. Vasan, Nigel W. Rayner, Alex P. Reiner, M. Revilla, Neil R. Robertson, Nicola Santoro, Claudia Schurmann, Wing Yee So, Heather M. Stringham, Tim M. Strom, Claudia H. T. Tam, Farook Thameem, Brian Tomlinson, Jason Torres, Russell P. Tracy, Rob M. van Dam, Marijana Vujković, Shuai Wang, Ryan Welch, Daniel R. Witte, Tien Yin Wong, Gil Atzmon, Nir Barzilai, John Blangero, Lori L. Bonnycastle, Donald W. Bowden, John C. Chambers, Edmund Chan, Ching‐Yu Cheng, Yoon Cho Shin, Francis S. Collins, Paul S. de Vries, Ravindranath Duggirala, Benjamin Gläser, Clicerio González, Ma Elena Gonzalez, Leif Groop, Jaspal S. Kooner, Soo Heon Kwak, Markku Laakso, K. Peter R. Nilsson, Timothy D. Spector, E Shyong Tai, Jaakko Tuomilehto, James Wilson, Carlos A. Aguilar‐Salinas, Erwin Böttinger, Brian Burke, David J. Carey, Juliana C.N. Chan, Josée Dupuis, Philippe Frossard, Susan R. Heckbert, Mi Yeong Hwang, Young Jin Kim, H. Lester Kirchner, Jong‐Young Lee, Juyoung Lee, Ruth J. F. Loos, Ronald C.W., Andrew D. Morris, Christopher J. O’Donnell, James S. Pankow, Kyong Soo Park, Asif Rasheed, Danish Saleheen, Xueling Sim, Kerrin S. Small, Yik Ying Teo, Christopher A. Haiman, Craig L. Hanis, Brian E. Henderson, Lorena Orozco, Teresa Tusié‐Luna, Frederick E. Dewey, Aris Baras, Christian Gieger, Thomas Meitinger, Konstantin Strauch, Leslie Lange, Niels Grarup, Torben Hansen, Oluf Pedersen, Philip Zeitler, Dana Dabelea, Gonçalo R. Abecasis, Graeme I. Bell, Nancy J. Cox, Mark Seielstad, Robert Sladek, James B. Meigs, Jerome I. Rotter, David Altshuler, Noёl P Burtt, Laura J. Scott, Andrew P. Morris, José C. Florez, Mark I. McCarthy, Michael Boehnke

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2018
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenetic Associations and Epidemiology
Canadian institutionsMcGill UniversityMcGill University and Génome Québec Innovation Centre
FundersNational Institutes of Health
KeywordsExome sequencingExomeGeneticsNonsynonymous substitutionGeneBiologyGenetic associationAlleleCandidate geneAllele frequencyComputational biologyBioinformaticsGenotypeMutationSingle-nucleotide polymorphismGenome

Abstract

fetched live from OpenAlex

Abstract Protein-coding genetic variants that strongly affect disease risk can provide important clues into disease pathogenesis. Here we report an exome sequence analysis of 20,791 type 2 diabetes (T2D) cases and 24,440 controls from five ancestries. We identify rare (minor allele frequency<0.5%) variant gene-level associations in (a) three genes at exome-wide significance, including a T2D-protective series of >30 SLC30A8 alleles, and (b) within 12 gene sets, including those corresponding to T2D drug targets ( p =6.1×10 −3 ) and candidate genes from knockout mice ( p =5.2×10 −3 ). Within our study, the strongest T2D rare variant gene-level signals explain at most 25% of the heritability of the strongest common single-variant signals, and the rare variant gene-level effect sizes we observe in established T2D drug targets will require 110K-180K sequenced cases to exceed exome-wide significance. To help prioritize genes using associations from current smaller sample sizes, we present a Bayesian framework to recalibrate association p -values as posterior probabilities of association, estimating that reaching p <0.05 ( p <0.005) in our study increases the odds of causal T2D association for a nonsynonymous variant by a factor of 1.8 (5.3). To help guide target or gene prioritization efforts, our data are freely available for analysis at www.type2diabetesgenetics.org .

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.004
metaresearch head score (Gemma)0.012
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.019

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0040.012
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.001
Research integrity0.0010.000
Insufficient payload (model declined to judge)0.0030.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.234
Teacher spread0.218 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations4
Published2018
Admission routes1
Has abstractyes

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