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A Novel Role for the PKG‐encoding <i>foraging</i> Gene in the Fly ‘Pancreas’ of <i>Drosophila melanogaster</i>

2016· article· en· W2889996834 on OpenAlexaff
Bryon N. Hughson, Anders Vesterberg, Marla B. Sokolowski

Bibliographic record

VenueThe FASEB Journal · 2016
Typearticle
Languageen
FieldNeuroscience
TopicNeurobiology and Insect Physiology Research
Canadian institutionsUniversity of Toronto
Fundersnot available
KeywordsDrosophila melanogasterBiologyAdipokinetic hormoneTransgeneMelanogasterGeneLipid metabolismCell biologySecretionGenetically modified mouseInternal medicineEndocrinologyGenetics

Abstract

fetched live from OpenAlex

The Drosophila melanogaster corpora cardiaca (CC) is functionally homologous to the mammalian pancreatic alpha cells and anterior pituitary gland. The CC is the site of biosynthesis and secretion of the fly glucagon homolog, adipokinetic hormone (AKH). The foraging gene ( for ) in D. melanogaster encodes a cGMP‐dependent protein kinase (PKG) and is evolutionarily conserved across taxa. for has a well‐established role in the regulation of feeding behavior and metabolism. Here we identify a CC‐specific role for for in the regulation of adult feeding behavior, lipid metabolism, and starvation stress response. The expression of transgenic for ‐RNAi constructs specifically in the CC significantly decreases adult whole body lipid content and starvation resistance, and increases adult feeding behavior. Immunohistochemistry data suggest that the transgenic manipulation of for in the CC alters the secretion of AKH. By restricting the CC‐specific transgenic manipulation of for to precise developmental periods, we demonstrate that the effects on adult metabolism and feeding behavior have their origin in juvenile life. These data demonstrate a novel role for foraging and PKG in the CC, and begin to elucidate a putative mechanism whereby juvenile experience can influence adult metabolism and health outcomes.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.049
GPT teacher head0.286
Teacher spread0.238 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2016
Admission routes1
Has abstractyes

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