Normal bystander keratinocytes protected by oleic acid against low dose radiation induced factor’s
Bibliographic record
Abstract
To investigate the protective role of Oleic acid against the cytotoxic mediators present in the low dose irradiated cell conditioned medium (ICCM) to unirradiated normal human keratinocyte cell line (HaCaT). Cell survival was investigated using the clonogenic assay, media transfer of HaCat cells exposed to low-LET γ radiation (0.5Gy) to by stander HaCat cells treated with oleic acid. The free radical scavenging property of oleic acid was performed by DCHFDA method and magnitude of lipid peroxidation was estimated with cisparineric acid as a probe using a fluorescence spectrometer. Mitochondrial membrane potential (Δψm) was observed using DiOC6 as a sensitive probe visualized under a confocal microscope. Based on clonogenic assay, it was found that a significant reduction in clonogenic survival was seen in unirradiated cells exposed to low dose ICCM. Results have further shown that HaCaT cells pretreated with oleic acid before the medium transfer showed an increased percentage of clonogenic survival suggesting protection of cells against the ICCM factors. Free radical scavenging activity was also reported in cell membrane and mitochondria. A correlation between the antioxidant properties of OA and an increase in clonogenic survival was seen. These results showed that oleic acid was protecting normal cells from indirect low-dose γ irradiated cells. It seems that ROS were involved in the mechanism of bystander effect which was significantly suppressed when OA was present.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".