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Record W2892861319 · doi:10.1002/ep.13000

Hydrolysis of oils in the Wadi Hanifah River in Saudi Arabia by free and immobilized <i>Staphylococcus aureus</i> ALA1 lipase

2018· article· en· W2892861319 on OpenAlex
Abir Ben Bacha, Islem Abid, Imededdine Arbi Nehdi, Habib Horchani

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueEnvironmental Progress & Sustainable Energy · 2018
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicEnzyme Catalysis and Immobilization
Canadian institutionsCégep de Rivière-du-Loup
FundersKing Saud University
KeywordsLipaseChemistryChromatographyHydrolysisWastewaterIncubationImmobilized enzymeBioremediationAdsorptionEnzymeBiodegradationEnzyme assayBiochemistryOrganic chemistryBiologyContaminationEnvironmental engineeringEnvironmental scienceEcology

Abstract

fetched live from OpenAlex

This study describes the biochemical properties of Staphylococcus aureus lipase immobilized by physical adsorption to assess its role in the biodegradation of oils in the Wadi Hanifah River. After optimization of the immobilization conditions, the recovered enzyme activity was 95% with appreciable increase in stability. The immobilized and free lipase retained 70% and only 10% of the initial activity after a 60‐min incubation at 80 °C, respectively. More than ~40% residual activity remained after 48 h of incubation at pH5 to 11. The immobilized enzyme retained 100% of the initial activity when used for four cycles and 42% of initial activity when stored at 25 °C for 120 days. Enzyme stability was enhanced in the presence of inactivating agents including β‐mercaptoethanol, SDS, EDTA, and Co 2+ . The bioremediation potential of lipid‐rich wastewater by the immobilized and free lipases was explored by analyses of chemical oxygen demand and lipid content. Both free and immobilized lipases efficiently hydrolyzed all oils tested and organic matter present in the wastewater. Overall, the results indicate the potential of immobilized S. aureus lipase in biological wastewater treatment and offer new options for several industrial applications. © 2018 American Institute of Chemical Engineers Environ Prog, 38:e13000, 2019

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.041
Threshold uncertainty score0.725

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.002
GPT teacher head0.193
Teacher spread0.191 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it