Effects of exogenous salicylic acid on accumulation of camptothecin and gene expression in <i>Camptotheca acuminata</i>
Bibliographic record
Abstract
Camptotheca acuminata Decne (Nyssaceae) is major natural source of camptothecin, an anticancer drug widely used for clinic therapy. Previous works have shown that many plant hormones or elicitors could regulate camptothecin biosynthesis, but few reports have examined sustainable effects of these plant hormones on plants for producing camptothecin. In this work, seedlings obtained from in vitro rapid propagation were used for investigating the sustainable effect of salicylic acid (SA) on transplanted C. acuminata tissues, especially leaves, for camptothecin biosynthesis. Our results indicate that exogenous SA could continuously induce the expression of iridoids pathway genes in C. acuminata leaves for promoting camptothecin production. High expression of iridoids pathway genes still could be observed in bioactive young leaves 180 days after seedlings had been transplanted to soil. In C. acuminata, iridoids pathway genes are expressed at very low levels or are not detectable in old leaves, resulting in decreasing production of camptothecin with leaf development. Interestingly, the expression of these genes could be detected clearly in old leaves of transplanted C. acuminata pretreated with 10 μmol·L–1 of SA during the in vitro rapid propagation phase, which indicates the particular function of SA for a sustainable effect on maintaining relatively high expression levels of iridoids pathway genes for camptothecin biosynthesis.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".