Abstract 201: Neuroprotective Effect of Hypothermic Total Liquid Ventilation in Newborn Piglets Subjected to Neonatal Cardiac Arrest
Bibliographic record
Abstract
Introduction: Hypothermic total liquid ventilation (TLV) has been shown to be highly protective in adult models of cardiac arrest. Hypothesis: TLV could also provide benefits through ultrafast cooling during neonatal cardiac arrest and subsequent hypoxic-ischemic encephalopathy in piglets. Methods: Newborn piglets were anesthetized and submitted to 30 min of hypoxia (FiO 2 =10%) followed by 7 min of asphyxia. After resucitation, animals underwent either normothermic follow-up (38°C; Control, n=6) or ultra-fast cooling by TLV (TLV, n=6). TLV was implemented during 30 min for cooling induction and hypothermia was further maintained during 3 h using external techniques before rewarming. A third group of piglets was submitted to a Sham procedure (n=4) with neither hypoxia nor ischemia. All groups underwent mechanical ventilation during 6-8 h before awakening. Survival and neurological dysfunction were assessed during 7 days. Animals were then euthanized and histological brain damages were assessed (fluorojade-C staining). Results: After hypoxia-ischemia, Control and TLV animals presented marked metabolic acidosis and lactate levels elevation as compared to Sham (e.g., pH=6.91±0.05, 6.93±0.02 vs 7.33±0.05 at t=30 min after ischemia in Control, TLV and Sham, respectively; p<0.05). Despite a trend toward reduced mortality, survival was not significantly different between TLV and Control groups at the end of the survival follow-up (survival = 0/6, 3/6 and 3/4 in Control, TLV and Sham groups). Nevertheless, neurological dysfunction was significantly reduced in TLV as compared to Control (median scores = 23% vs 87 at day 2, respectively; Sham value = 0%). Blood levels of the brain injury biomarker protein S100B were also reduced at the time of euthanasia in TLV vs Control. Fluoro-jade C staining showed a significantly lower number of degenerating neurons in TLV vs Control (e.g., medians = 17 vs 39 degenerating neurons per field in caudate nucleus, respectively; Sham value = 1). Conclusion: Hypothermic TLV improves neurological recovery in a swine model of neonatal cardiac arrest and hypoxic-ischemic encephalopathy when started early after resuscitation.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".