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Record W2897914393 · doi:10.1083/jcb.201801122

EHD2 is a mechanotransducer connecting caveolae dynamics with gene transcription

2018· article· en· W2897914393 on OpenAlexfundno aff
Stéphanie Torrino, Weiwei Shen, Cédric M. Blouin, Christine Viaris de Lesegno, Pierre Bost, Alexandre Grassart, D Köster, Cesar Augusto Valades‐Cruz, Valérie Chambon, Ludger Johannes, Paolo Pierobon, Vassili Soumelis, Catherine Coirault, Stéphane Vassilopoulos, Christophe Lamaze

Bibliographic record

VenueThe Journal of Cell Biology · 2018
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCaveolin-1 and cellular processes
Canadian institutionsnot available
FundersHorizon 2020 Framework ProgrammeH2020 European Research CouncilCentre National de la Recherche ScientifiqueFondation de FranceInstitut National Du CancerInnovative Research Group Project of the National Natural Science Foundation of ChinaInstitut National de la Santé et de la Recherche MédicaleInstitut CurieMarie CurieMinisterio de Economía y CompetitividadInfrastructures en Biologie Santé et AgronomieLabex CelTisPhyBioGordon and Betty Moore FoundationHuman Frontier Science ProgramInstitut national de la recherche scientifiqueAssociation Française contre les MyopathiesFondation ARC pour la Recherche sur le CancerAgence Nationale de la RechercheInstitute of Cancer ResearchLabexHoward Hughes Medical Institute
KeywordsCaveolaeCell biologyMechanotransductionTranscription factorBiologyChemistryGeneSignal transductionGenetics

Abstract

fetched live from OpenAlex

Caveolae are small invaginated pits that function as dynamic mechanosensors to buffer tension variations at the plasma membrane. Here we show that under mechanical stress, the EHD2 ATPase is rapidly released from caveolae, SUMOylated, and translocated to the nucleus, where it regulates the transcription of several genes including those coding for caveolae constituents. We also found that EHD2 is required to maintain the caveolae reservoir at the plasma membrane during the variations of membrane tension induced by mechanical stress. Metal-replica electron microscopy of breast cancer cells lacking EHD2 revealed a complete absence of caveolae and a lack of gene regulation under mechanical stress. Expressing EHD2 was sufficient to restore both functions in these cells. Our findings therefore define EHD2 as a central player in mechanotransduction connecting the disassembly of the caveolae reservoir with the regulation of gene transcription under mechanical stress.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.223
Teacher spread0.216 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations86
Published2018
Admission routes1
Has abstractyes

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