A Simple DNAzyme‐Based Fluorescent Assay for <i>Klebsiella pneumoniae</i>
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Bibliographic record
Abstract
Abstract Pathogenic bacteria pose a serious threat to public health, and the rapid and cost‐effective detection of such bacteria remains a major challenge. Herein, we present a DNAzyme‐based fluorescent paper sensor for Klebsiella pneumoniae . The DNAzyme was generated by an in vitro selection technique to cleave a fluorogenic DNA–RNA chimeric substrate in the presence of K . pneumoniae . The DNAzyme was printed on a paper substrate in a 96‐well format to serve as mix‐and‐read fluorescent assay that exhibits a limit of detection (LOD) 10 5 CFUs mL −1 . Evaluated with 20 strains of clinical bacterial isolates, the DNAzyme produced the desired fluorescence signal with the samples of K . pneumoniae, regardless of their source or drug resistance. The assay is simple to use, rapid, inexpensive, and avoids the complex procedures of sample preparation and equipment. We believe that this DNAzyme‐based fluorescent assay has potential for practical applications to identify K . pneumoniae .
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it