A Novel Molecular Diagnostic Device Using Solid-Phase PCR on a Hotwire Optical Fibre Grating
Bibliographic record
Abstract
The usage of fibre optic technologies presents the possibility of high volume manufacturing at low costs when used with novel diagnostic assays.In this thesis, the technologies of hotwire fibre optic devices are combined with in-fibre Bragg gratings to produce a platform compatible with the developing field of Solid Phase PCR (SP-PCR).Here we demonstrate that the DNA coating of such devices can be performed with economical UV-cross-linking.In the process of validating the platform for SP-PCR usage we have also created a novel assay for monitoring real-time polymerase enzyme activity on solidsurface-attached DNA sequences.The device can function with SP-PCR in real-time through its usage of Total Internal Reflection Fluorescence (TIRF).Our device demonstrates the ability to spatially separate several reactions on a single fibre, representing a novel fibre application, and has the potential to reliably increase the speed and multiplexability of the SP-PCR reactions.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".