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Record W2904879791 · doi:10.1038/s41598-018-35970-8

An automated bacterial concentration and recovery system for pre-enrichment required in rapid Escherichia coli detection

2018· article· en· W2904879791 on OpenAlexafffund
Yushan Zhang, Chang‐Qing Xu, Tianyi Guo, Lingcheng Hong

Bibliographic record

VenueScientific Reports · 2018
Typearticle
Languageen
FieldEngineering
TopicBiosensors and Analytical Detection
Canadian institutionsMcMaster University
FundersNatural Sciences and Engineering Research Council of CanadaCanadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada
KeywordsEscherichia coliEscherichia coli ProteinsMicrobiologyChemistryBiologyBiochemistryGene

Abstract

fetched live from OpenAlex

One of the biggest challenges in rapid low concentration bacterial detection is the pre-concentration or pre-enrichment, which aims to increase bacteria concentration and reduce sample volume for easy bacterial detection. In practical bacterial detection, large-volume water samples with a pathogenic bacterial concentration of less than 1 CFU/mL have to be tested rapidly. The reported biosensors either have insufficient detection limit or have limited capability of handling a sufficiently large water sample. Therefore, a high-performance automated pre-enrichment process is strongly demanded in rapid practical bacterial detection. In this paper, a practical high performance automated bacterial concentration and recovery system (ABCRS) based on the combination of a ceramic membrane and tangential flow filtration technique was presented with short processing time (less than one hour), low pre-enrichment limit (≤0.005 CFU/mL), high concentration ratio (≥ 500), high recovery efficiency (~ 90%), and small final retentate volume (≤ 5 mL).

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.520
Threshold uncertainty score0.385

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.238
Teacher spread0.229 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations36
Published2018
Admission routes2
Has abstractyes

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