Towards High Throughput Electroporation of Zebrafish Folicles
Bibliographic record
Abstract
This study presents a new approach for delivery of molecules into the cell Layers of Zebrafish Follicle which can be crucial for understanding of ovarian development and the treatment of ovarian diseases. Zebrafish follicles are used as a model of ovarian development. These follicles consists of an oocyte surrounded by two thin layers of cells called theca and granulosa cell. Electroporation is a non-invasive method widely used for transferring molecules into cells for various applications including stem cell based tissue construction and gene therapy. Despite broad advantages of electroporation, no reports have been released for application of drug delivery into Zebrafish follicle and Follicle cells. This paper is the first to discuss the advantage of electroporation for the delivery of biomolecules into the follicle and follicle cells. Herein we demonstrate and discuss the advantage of electroporation for the molecule delivery into follicle and follicle cells using Propidium Iodide (PI) and green fluorescence protein (GFP).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".