Simultaneous two-photon and three-photon microscopy imaging with a dual-wavelength Er-doped fiber laser
Bibliographic record
Abstract
A multimodal multiphoton microscopy (MPM) is developed to acquire two-photon and three-photon signals simultaneously, including two-photon excitation fluorescence (TPEF), second harmonic generation (SHG), and third harmonic generation (THG). We have developed a miniature multimodal MPM system based on a dual-wavelength Erdoped fiber laser, which includes the fundamental pulse at 1580 nm to excite THG and the frequency-doubled pulse at 790 nm to excite TPEF and SHG. The laser is coupled by a single mode fiber into a miniature MPM imaging probe. Label-free imaging by TPEF, SHG, and THG are demonstrated on biological samples, obtained from intrinsic fluorophores, collagen, lipid and interface.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".