Abstract P227: Mapping of Chromosome 2 Regions Linked to Vascular Inflammation Using Congenic Rats
Bibliographic record
Abstract
Objective: Chromosome 2 (chr2) introgression from normotensive Brown Norway rats (BN) into hypertensive Dahl salt sensitive (SS) background (consomic SS2 BN ) reduced blood pressure (BP) and vascular inflammation under a normal-salt diet. Mapping of chr2 using congenic rats revealed that the distal portion of BN chr2 (SS2 BN a) but not the middle segment (SS2 BN b) on the SS background under a normal-salt diet contains anti-inflammatory genes. However, the role of chr2 in the regulation of vascular inflammation under a high-salt diet (HSD) remains unknown. We hypothesized that SS2 BN a but not SS2 BN b rats would have reduced vascular inflammation under HSD. Design and method: Four-to-6 week old male SS, SS2 BN a and SS2 BN b rats were fed a HSD (4% NaCl) for 8 weeks or until they developed a stroke as manifested by seizures. Vascular remodeling was assessed in mesenteric arteries (MA) using pressurized myography. Reactive oxygen species (ROS) production by dihydroethidium staining, vascular cell adhesion molecule (VCAM)-1 expression and CD3 + T cell infiltration by immunofluorescence were determined in aorta or perivascular adipose tissue (PVAT). BP was measured by telemetry after 6 weeks of HSD. Results: Systolic BP tended to be higher in SS2 BN b compared to SS (185±8 vs 168±5 mmHg). The incidence of seizures was 3.9-fold higher in SS2 BN b compared to SS (12/36 vs 3/35 rats ( P <0.05). MA media/lumen was 1.3-fold higher in SS2 BN a compared to SS (10.6±0.9 vs 7.9±0.4%, P <0.01). PVAT ROS production was 1.8-fold higher in SS2 BN a compared to SS (5.6±0.8 vs 3.2±0.1 relative fluorescence units [RFU]/μm 2 , P <0.01) and tended to be lower in SS2 BN b (2.1±0.4 vs 3.2±0.1 RFU/μm 2 ). Aortic VCAM-1 was increased 2.1-fold in SS2 BN a compared to SS (2.6±0.5 vs 1.2±0.3 RFU/μm 2 , P <0.05). Aortic PVAT CD3 + T cell infiltration was 55% lower in SS2 BN b compared to SS (17±4 vs 38±4 cells/mm 2 , P <0.05). Conclusions: Unexpectedly, SS2 BN a rats present increased vascular injury under HSD. The absence of vascular inflammation and remodeling in SS2 BN b rats despite slightly higher BP seems maladaptive and may explain the increased incidence of stroke.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.002 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.011 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".