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Record W2923371440 · doi:10.1002/eji.201847897

Expression of IL‐7Rα and KLRG1 defines functionally distinct CD8 <sup>+</sup> T‐cell populations in humans

2019· article· en· W2923371440 on OpenAlexaff
Ester B. M. Remmerswaal, Pleun Hombrink, Benjamin Nota, Hanspeter Pircher, Ineke J. M. ten Berge, René A. W. van Lier, Michiel C. van Aalderen

Bibliographic record

VenueEuropean Journal of Immunology · 2019
Typearticle
Languageen
FieldImmunology and Microbiology
TopicT-cell and B-cell Immunology
Canadian institutionsInstitute of Infection and Immunity
FundersNierstichting
KeywordsBiologyImmunologyPopulationCD8Cytotoxic T cellLymphEffectorGranzyme BIL-2 receptorCell biologyT cellImmune systemGeneticsPathologyMedicineIn vitro

Abstract

fetched live from OpenAlex

Abstract During acute viral infections in mice, IL‐7Rα and KLRG1 together are used to distinguish the short‐lived effector cells (SLEC; IL‐7Rα lo KLRG hi ) from the precursors of persisting memory cells (MPEC; IL‐7Rα hi KLRG1 lo ). We here show that these markers can be used to define distinct subsets in the circulation and lymph nodes during the acute phase and in “steady state” in humans. In contrast to the T cells in the circulation, T cells derived from lymph nodes hardly contain any KLRG1‐expressing cells. The four populations defined by IL‐7Rα and KLRG1 differ markedly in transcription factor, granzyme and chemokine receptor expression. When studying renal transplant recipients experiencing a primary hCMV and EBV infection, we also found that after viral control, during latency, Ki‐67‐negative SLEC can be found in the peripheral blood in considerable numbers. Thus, combined analyses of IL‐7Rα and KLRG1 expression on human herpes virus‐specific CD8 + T cells can be used to separate functionally distinct subsets in humans. As a noncycling IL‐7Rα lo KLRG1 hi population is abundant in healthy humans, we conclude that this combination of markers not only defines short‐lived effector cells during the acute response but also stable effector cells that are formed and remain present during latent herpes infections.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.721
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.218
Teacher spread0.201 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations48
Published2019
Admission routes1
Has abstractyes

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