Susceptibility of Different Instars of Striacosta albicosta (Lepidoptera: Noctuidae) to Vip3A, a Bacillus thuringiensis (Bacillaceae: Bacillales) Protein
Bibliographic record
Abstract
Striacosta albicosta (Smith) (Lepidoptera: Noctuidae) is an important pest of corn, Zea mays L. in the Great Lakes region, which can be controlled by transgenic corn expressing Vip3A protein from Bacillus thuringiensis. To inform insect resistance management, the susceptibility, survival, and development of first, third, and fifth instar S. albicosta to Vip3A was determined using protein-overlay and corn tissue bioassays. Tissue bioassays were also used to determine the quantity of corn tissues with and without Vip3A-expression consumed by various instars. In diet bioassays, third and fifth instars were significantly less susceptible to Vip3A compared with first instars; however, no significant difference was observed in susceptibility of older instars. In tissue bioassays, survival was lowest for larvae fed Vip3A-expressing tissues, ranging from 0 to 21%, however, developmental measures of larvae fed Vip3A-expressing tissues did not differ from those fed artificial diet or tissues of other Bt events. Consumption of Vip3A × Cry1Ab tissues did not differ from that of Cry1Ab for each instar. Estimated Vip3A exposure of first instars ranged from 3 to 57 times higher than the concentration required for 99% mortality (LC99) based on the product of the reported Vip3A expression in transgenic corn tissues and the consumption observed in tissue bioassays; however, the estimated exposure of third and fifth instars to Vip3A was lower than their respective LC99. These findings suggest that first instar S. albicosta maybe exposed to a high dose of Vip3A under field conditions; however, Vip3A-expression in corn may not be high dose against older instars, increasing the risk of resistance development.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".