Testing of the mutagenic potential of N-(γ-aminobuturyl)-1-aza-15-crown-5 hydrochloride in Ames test microplate modification
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
In recent years, studies in the field of chemical mutagenesis have undergone significant development, due to the introduction of a large number of different chemicals and scientific advances in the creation and use of new test systems, allowing a complete assessment of both mutagens themselves and their metabolites. The aim of the work was to determine possible induction of gene mutations under influence of hydrochloride N-(γ-aminobuturil)-1-aza-4,7,10,13-tetraozacyclopentadecan (TOCPD), which has nootropic, anxiolytic and anticonvulsant activity. The ability of TOCPD to induce gene mutations was evaluated in Ames test on strains Salmonella typhimurium ТА 98 (frame shift mutations) and ТА 100 (substitution point mutations). The compound was used at concentrations of 10, 100, 250, 500 and 1000 μg/ml. Standard mutagens were 2-nitrofluoren for Salmonella typhimurium ТА 98 and sodium azide for Salmonella typhimurium ТА 100 in test without metabolic activation. In an activation variant a microsomal activating mixture was used (S9 mix). In tests with activation for both strains, 2-aminoantracene was used. The µAmes kit, Moltox (USA) and Muta-ChromoPlate kit (Canada) were used in the work. The results were evaluated by the number of wells with mutated cells with medium color changing from purple to yellow. The obtained data showed that in the control and according to the action of corresponding mutagens, the percentage of wells with mutated cells corresponded to the standard parameters determined by protocol of the microplate test. For the action of TOCPD compound, no gene mutations were detected in both S. typhimurium ТА 98 and ТА 100 strains within the concentrations used.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it