Heat shock Factor 8 (hsf8) introduced into soybean mediated-Agrobacterium transformation
Bibliographic record
Abstract
Plant transformation is an important approach in genetic engineering breeding and identification of gene function. Objective of this study is to transfer heat shock factor8 (hsf8) gene into soybean variety and improve expression of target gene hsp70 in order to enhance heat tolerance of soybean. In this experiment, hsf8 was transferred into soybean cultivar Kexin3 through Agrobacterium-mediated method. Thirteen kanamycin-resistant transgenic plants were obtained, and these plants were examined by PCR amplification, and nine of them showed positive signals, indicating that hsf8 gene wa sintegrated into genomes of these nine transgenic plants. The frequency of soybean transformants in this experiment was 2.39%. In addition, the effects of improve the frequence of transformation were discussed, including the suitable sterilization method of the seed and genotype of soybean.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".