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Record W2948226244

Heat shock Factor 8 (hsf8) introduced into soybean mediated-Agrobacterium transformation

2004· article· en· W2948226244 on OpenAlexvenueno aff
Qingnu Yin, Huiying Lü, Baoge Zhu, Jing Zhang

Bibliographic record

Venue分子植物育种 · 2004
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicPlant Genetic and Mutation Studies
Canadian institutionsnot available
Fundersnot available
KeywordsAgrobacteriumBiologyGenetically modified cropsTransformation (genetics)KanamycinGeneCultivarGenomeTransgeneBiotechnologyGeneticsBotanyHorticulture
DOInot available

Abstract

fetched live from OpenAlex

Plant transformation is an important approach in genetic engineering breeding and identification of gene function. Objective of this study is to transfer heat shock factor8 (hsf8) gene into soybean variety and improve expression of target gene hsp70 in order to enhance heat tolerance of soybean. In this experiment, hsf8 was transferred into soybean cultivar Kexin3 through Agrobacterium-mediated method. Thirteen kanamycin-resistant transgenic plants were obtained, and these plants were examined by PCR amplification, and nine of them showed positive signals, indicating that hsf8 gene wa sintegrated into genomes of these nine transgenic plants. The frequency of soybean transformants in this experiment was 2.39%. In addition, the effects of improve the frequence of transformation were discussed, including the suitable sterilization method of the seed and genotype of soybean.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.676
Threshold uncertainty score0.270

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.214
Teacher spread0.198 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2004
Admission routes1
Has abstractyes

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