Side effects of immune response of Colorado potato beetle, Leptinotarsa decemlineata against the entomopathogenic nematode, Steinernema carpocapsae infection
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Entomopathogenic nematodes (EPNs) are lethal pathogens of agricultural insect pests. Little is known about their sublethal effects on the insect hosts. The lethal effects of Steinernema carpocapsae on fourth instar larvae of Colorado potato beetle (CPB), Leptinotarsa decemlineata were detected using soil infection and direct injection of the nematode into the hemocel. LC20 and LC80 values of 7.8 (3.0 - 13.4) infective juveniles (IJs) and 126.7 (91-206.7) IJs were obtained for the soil application method and 10.2 (8.7 - 11.4) IJs and 22.7 (19.73 - 28.0) IJs for direct injection, respecitvely. Sublethal effects of S. carpocapsae on last instar larvae and subsequent surviving adults and phenoloxidase (PO) activity in hemolymph of nematode-injected last instar larvae were investigated. Sublethal effects included adult cuticular discoloration, deformation of the wings, legs and antenna and decreased fertilized egg production in females. Considering cuticular discoloration in most treated insects, it is hypothesized that production of PO in the insect larvae infected with an entomopathogenic nematode, S. carpocapsae might have costs for surviving adult insects. PO specific activity in CPB against S. carpocapsae generally increased up to 48 h post injection. Here in, the sublethal effects are discussed as a potential tread-offs of PO production in nematode-injected insects.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.004 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.003 | 0.001 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it