Effects of canopy cover and pH on polyketide synthase gene transcription in Cladonia stygia and the polyketides produced in natural conditions.
Bibliographic record
Abstract
Polyketide synthase (PKS) genes encode large multidomain enzymes that synthesize polyketides (secondary metabolites) but little is known about their biosynthesis. The goals of this study were to identify PKS genes in the Cladonia rangiferina genome in order to target PKS genes in Cladonia stygia, and to compare PKS gene transcription and polyketide production in Cladonia stygia collected from two geologically different locations in Manitoba. The Antibiotic and Secondary Metabolite Analysis Shell (antiSMASH) program identified 41 secondary metabolite gene clusters consisting of 15 Type 1 PKS genes but only two Non-Reducing Type 1 PKS clusters. The significant difference in the concentration of the polyketides, atranorin and fumarprotocetraric acid, between the two locations corresponded with gene expression using the 21 Beta-Ketoacyl Synthase (p=0.040) and 26 Thioesterase domain (p=0.081), suggesting that these PKS domains may play a role in the production of fumarprotocetraric acid and atranorin, respectively, in the C. stygia thallus.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".