PM314. Knockdown Piccolo suppressed Methamphetamine-induced behavioral changes and dopamine release in the nucleus accumbens of mice
Bibliographic record
Abstract
Abstract The Piccolo gene was identified as one increased molecular in nucleus accumbens (NAc) of mice which treated with methamphetamine (METH) continuously. Piccolo is originally reported as a Ca2+ sensor for regulating release of insulin in pancreas β cell. Piccolo is a protein of the pre-synapse, and it is comprised of domains such as PDZ, C2A or C2B mainly. The PDZ domain interacts with other pre-synapse proteins comprised of active zone. The C2A domain is including Ca2+ binding site, and it is considered that spatial structure changes in Ca2+-binding presence. It is reported that Piccolo plays an important role in the neuronal synapse. However, function of Piccolo gene in NAc remains unclear in the drug dependence. Therefore, we investigated the physiological function of Piccolo in the NAc of mice, which were received METH treatment. To clarify of Piccolo in the NAc, we characterized the NAc-specific knockdown mice (mi Piccolo mice) by adeno-associated virus (AAV) vector including Piccolo miRNA. METH-induced locomotor activity of mi Piccolo mice was reduced compared with that of AAV-mock mice (Mock mice). The mi Piccolo mice suppressed the METH-induced CPP formation. In the microdialysis test, the mi Piccolo mice exhibited lower dopamine basal levels and tendency to decrease METH-potentiated dopamine release. These results suggested that Piccolo in the NAc regulates dopaminergic neuronal systems and METH-dependence related-behaviors.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".