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Record W2971525299 · doi:10.1038/s41467-019-11884-5

YAP1 subgroup supratentorial ependymoma requires TEAD and nuclear factor I-mediated transcriptional programmes for tumorigenesis

2019· article· en· W2971525299 on OpenAlexfundno aff
Kristian W. Pajtler, Yiju Wei, Konstantin Okonechnikov, Patricia B. G. Silva, Mikaella Vouri, Lei Zhang, Sebastian Brabetz, Laura Sieber, Melissa Gulley, Monika Mauermann, Tatjana Wedig, Norman Mack, Yuka Imamura Kawasawa, Tanvi Sharma, Marc Zuckermann, Felipe Andreiuolo, Eric C. Holland, Kendra K. Maaß, Huiqin Körkel-Qu, Hai‐Kun Liu, Felix Sahm, David Capper, Jens Bunt, Linda J. Richards, David Jones, Andrey Korshunov, Lukas Chávez, Peter Lichter, Mikio Hoshino, Stefan M. Pfister, Marcel Kool, Wei Li, Daisuke Kawauchi

Bibliographic record

VenueNature Communications · 2019
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicHippo pathway signaling and YAP/TAZ
Canadian institutionsnot available
FundersNational Cancer InstituteUniversity at BuffaloUniversity of Texas MD Anderson Cancer CenterInstitute of GeneticsNational Institutes of HealthBundesministerium für Bildung und ForschungFour DiamondsUniversity of PennsylvaniaCERNDeutsche ForschungsgemeinschaftDeutsches KrebsforschungszentrumStichting Kinderen KankervrijPennsylvania State UniversityPenn State College of MedicineDeutsche KrebshilfeUniversity of Texas Southwestern Medical Center
KeywordsYAP1CarcinogenesisTranscription factorBiologyEpendymomaCancer researchChromatin immunoprecipitationCell biologyGeneticsCancerGeneMedicinePathologyGene expressionPromoter

Abstract

fetched live from OpenAlex

YAP1 fusion-positive supratentorial ependymomas predominantly occur in infants, but the molecular mechanisms of oncogenesis are unknown. Here we show YAP1-MAMLD1 fusions are sufficient to drive malignant transformation in mice, and the resulting tumors share histo-molecular characteristics of human ependymomas. Nuclear localization of YAP1-MAMLD1 protein is mediated by MAMLD1 and independent of YAP1-Ser127 phosphorylation. Chromatin immunoprecipitation-sequencing analyses of human YAP1-MAMLD1-positive ependymoma reveal enrichment of NFI and TEAD transcription factor binding site motifs in YAP1-bound regulatory elements, suggesting a role for these transcription factors in YAP1-MAMLD1-driven tumorigenesis. Mutation of the TEAD binding site in the YAP1 fusion or repression of NFI targets prevents tumor induction in mice. Together, these results demonstrate that the YAP1-MAMLD1 fusion functions as an oncogenic driver of ependymoma through recruitment of TEADs and NFIs, indicating a rationale for preclinical studies to block the interaction between YAP1 fusions and NFI and TEAD transcription factors.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.960
Threshold uncertainty score0.566

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.277
Teacher spread0.260 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations110
Published2019
Admission routes1
Has abstractyes

Explore more

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