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Record W2974871632 · doi:10.1039/c9sm00930b

Toward building a physical model for membrane selectivity of antimicrobial peptides: making a quantitative sense of the selectivity

2019· article· en· W2974871632 on OpenAlexafffund
Shokoofeh Nourbakhsh, Sattar Taheri-Araghi, Bae‐Yeun Ha

Bibliographic record

VenueSoft Matter · 2019
Typearticle
Languageen
FieldImmunology and Microbiology
TopicAntimicrobial Peptides and Activities
Canadian institutionsRegional Municipality of WaterlooUniversity of Waterloo
FundersNational Institute of General Medical SciencesNatural Sciences and Engineering Research Council of CanadaNational Institutes of Health
KeywordsSelectivityAntimicrobialAntimicrobial peptidesMembraneSense (electronics)ChemistryCombinatorial chemistryNanotechnologyBiophysicsMaterials scienceBiochemistryOrganic chemistryBiologyCatalysisPhysical chemistry

Abstract

fetched live from OpenAlex

Antimicrobial peptides (AMPs) are naturally-occurring peptide antibiotics. AMPs are typically cationic and utilize their electrostatic interactions with the bacterial membrane to selectively attack bacteria. The way they work has inspired a vigorous search for optimized peptides for fighting resistant bacteria. Here, we present a physical model of membrane selectivity of AMPs. The challenge for theoretical modeling of membrane-peptide systems arises from the simultaneous presence of several competing effects, including lipid demixing and peptide-peptide interactions on the membrane surface. We first examine critically a number of models of peptide-membrane interactions and map out one, which incorporates adequately these competing effects as well as the geometry of various regions in membranes, occupied by bound peptides, anionic lipids within the interaction range of each peptide, and those outside this range. This effort leads to a systematically-improved model for peptide selectivity. Using the model, we relate peptide's intrinsic (Ccell-independent) selectivity to an apparent, Ccell-dependent one, and clarify the relative roles of peptide parameters and cell densities in determining their selectivity. This relationship suggests that the selectivity is more sensitive to peptide parameters at low cell densities; as a result, the optimal peptide charge, at which the selectivity is maximized, increases with the cell density in such a manner that this notion becomes less meaningful at high cell densities.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.011
Threshold uncertainty score0.713

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.022
GPT teacher head0.269
Teacher spread0.248 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations6
Published2019
Admission routes2
Has abstractyes

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